Transient Gene Expression is an Effective Experimental Tool for the Research into the Fine Mechanisms of Plant Gene Function: Advantages, Limitations, and Solutions
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences (IPP RAS), Moscow 127276, Russia
Author to whom correspondence should be addressed.
Received: 10 July 2022
Revised: 31 August 2022
Accepted: 8 September 2022
Published: 11 September 2022
A large data array on plant gene expression accumulated thanks to comparative omic studies directs the efforts of researchers to the specific or fine effects of the target gene functions and, as a consequence, elaboration of relatively simple and concurrently effective approaches allowing for the insight into the physiological role of gene products. Numerous studies have convincingly demonstrated the efficacy of transient expression strategy for characterization of the plant gene functions. The review goals are (i) to consider the advantages and limitations of different plant systems and methods of transient expression used to find out the role of gene products; (ii) to summarize the current data on the use of the transient expression approaches for the insight into fine mechanisms underlying the gene function; and (iii) to outline the accomplishments in efficient transient expression of plant genes. In general, the review discusses the main and critical steps in each of the methods of transient gene expression in plants; areas of their application; main results obtained using plant objects; their contribution to our knowledge about the fine mechanisms of the plant gene functions underlying plant growth and development; and clarification of the mechanisms regulating complex metabolic pathways. View Full-Text
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Supplementary File 1:
ZIP-Document (ZIP, 1052 KiB)
Share and Cite
MDPI and ACS Style
Tyurin, A.A.; Suhorukova, A.V.; Kabardaeva, K.V.; Goldenkova-Pavlova, I.V. Transient Gene Expression is an Effective Experimental Tool for the Research into the Fine Mechanisms of Plant Gene Function: Advantages, Limitations, and Solutions.
9, 1187. https://doi.org/10.3390/plants9091187
Tyurin AA, Suhorukova AV, Kabardaeva KV, Goldenkova-Pavlova IV. Transient Gene Expression is an Effective Experimental Tool for the Research into the Fine Mechanisms of Plant Gene Function: Advantages, Limitations, and Solutions.
Plants. 2022; 9(9):1187. https://doi.org/10.3390/plants9091187
Tyurin, Alexander A., Alexandra V. Suhorukova, Ksenia V. Kabardaeva, and Irina V. Goldenkova-Pavlova. 2022. “Transient Gene Expression is an Effective Experimental Tool for the Research into the Fine Mechanisms of Plant Gene Function: Advantages, Limitations, and Solutions”
9, no. 9: 1187. https://doi.org/10.3390/plants9091187
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
Article Access Statistics
For more information on the journal statistics, click here.
Multiple requests from the same IP address are counted as one view.
Https Www Domain_9 Com Help Domain_9 Answer 1187